50mM phosphate buffer solution.

2% Papain standard solution.

 Papain standards solution with various concentration.

Obtaining 10 mmol of BAPA solutions.

Obtaining 1mmol (Working Reagent, WR) of BAPA solution

Obtaining 30% acetic acid solution.

Reaction of the Papain activity and Homogenous of the mixture.

 Stopping the reaction for the Papain activity and substrate.

Determination of the Papain content 

Obtaining the standard curve with difference concentration.

The value of their absorbance at the specific wavelength .                                                  

 The value of Papain content in the sample.