UV-VIS Papain Activity Measurement
- Product Code: 35452
UV-VIS Papain Activity Measurement
timeline Service Steps
| Step | Procedure | Expected Result |
|---|---|---|
|
1
|
50mM... |
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50mM phosphate buffer solution. |
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2
|
0.02g... |
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2% Papain standard solution. |
|
3
|
Dilute... |
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Papain standards solution with various concentration. |
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4
|
0.004349... |
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Obtaining 10 mmol of BAPA solutions. |
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5
|
Prepare... |
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Obtaining 1mmol (Working Reagent, WR) of BAPA solution |
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6
|
30ml... |
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Obtaining 30% acetic acid solution. |
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7
|
120... |
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Reaction of the Papain activity and Homogenous of the mixture. |
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8
|
The... |
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Stopping the reaction for the Papain activity and substrate. |
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9
|
... |
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Determination of the Papain content |
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10
|
Prepare... |
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Obtaining the standard curve with difference concentration. |
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11
|
Use... |
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The value of their absorbance at the specific wavelength . The value of Papain content in the sample. |
You will receive a report for the The value of Papain activity( U/g ) , the value of Papain concentration (ug/ml or %) fields when we provide this service
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