In Vitro SPF and UVA-PF Measurement by UV Spectrophotometry
- Product Code: 253469
In vitro UV spectrophotometric assay for Sun Protection Factor (SPF, 290-320 nm weighted) and UVA Protection Factor (UVA-PF, 320-400 nm) via ethanol dilution, sonication, filtration, and transmittance measurement in quartz cuvettes.
Sample Requirement:
Type: semi-solid (sunscreen cream/lotion)
Amount: 2 grams
description ภาพรวมบริการ
In vitro UV spectrophotometric assay for Sun Protection Factor (SPF, 290-320 nm weighted) and UVA Protection Factor (UVA-PF, 320-400 nm) via ethanol dilution, sonication, filtration, and transmittance measurement in quartz cuvettes.
Sample Requirement:
Type: semi-solid (sunscreen cream/lotion)
Amount: 2 grams
timeline ขั้นตอนการให้บริการ
| ขั้นตอน | ขั้นตอน | ผลลัพธ์ที่คาดหวัง |
|---|---|---|
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1
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Prepare 1... |
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Clear, colourless 1 % HCl solution (pH ≈ 2). |
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2
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Prepare 1... |
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Orange-brown clear reagent, stable 1 month in dark. |
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3
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Prepare 1... |
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Homogenous 1 mg mL⁻¹ stock (1 000 µg mL⁻¹). |
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4
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Prepare 100... |
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100 µg mL⁻¹ SA working solution. |
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5
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Prepare calibration... |
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Violet solutions of increasing intensity; expected absorbances ≈0–0.25 AU. |
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6
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Zero instrument:... |
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Baseline absorbance = 0.000 AU. |
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7
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Generate calibration... |
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Slope ≈ 0.0041 AU µg⁻¹ mL; R² ≥ 0.99. |
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8
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Prepare porcine... |
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Hydrated, intact skin squares free of fat |
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9
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Apply cleanser:... |
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Uniform foam layer covering test area. |
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10
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Rinse: Hold... |
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Visible foam removed; skin damp but not dripping. |
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11
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Collect residue:... |
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Swab visibly contacts whole surface; tip recovered intact. |
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12
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Extract: Add... |
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Clear methanolic extract containing SA. |
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13
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Develop colour... |
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Violet test solution; blank remains pale. |
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14
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Measure sample:... |
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Sample absorbance in 0.000–0.250 AU range. |
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15
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Calculate SA... |
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Numerical concentration of extract. |
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16
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Compute residue:... |
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Final residue value; expected < 2 µg cm⁻² for mild cleansers. |
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17
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Record &... |
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Complete dataset ready for QA review. |
คุณจะได้รับรายงานสำหรับ Sample absorbance at 525 nm (AU) , Residual SA on skin (µg cm⁻²) ฟิลด์เมื่อเราให้บริการนี้
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