This spectrophotometric method is used to quantify Ascorbyl Glucoside in a given sample. Ascorbyl Glucoside is a vitamin C derivative commonly used in skincare and pharmaceutical products for its stable, skin-benefiting properties. Accurately determining its concentration ensures product quality, efficacy, and compliance with formulation specifications. The assay uses UV-Vis spectrophotometry at 260 nm, where Ascorbyl Glucoside shows a strong absorbance. A calibration curve over a linear range of 0–35 µg/mL provides a reliable relationship between absorbance and concentration. By measuring sample absorbance under the same conditions and applying the linear equation (y = 0.0402x + 0.0436), the method allows precise calculation of Ascorbyl Glucoside content.