CHAPSO is widely used in biochemistry and molecular biology as a zwitterionic detergent. It is particularly effective in solubilizing membrane proteins without denaturing them, making it a valuable tool for studying protein structure and function. The chemical is often employed in the preparation of samples for X-ray crystallography and nuclear magnetic resonance (NMR) spectroscopy, where maintaining protein integrity is crucial. Additionally, CHAPSO is used in the formation of lipid bilayers and vesicles, aiding in the study of membrane dynamics and interactions. Its ability to stabilize proteins and lipids in aqueous solutions also makes it useful in various assays and experimental protocols.