Mouse WNT9A, Tag Free is primarily used in cell signaling research, particularly in studies involving the Wnt signaling pathway, which plays a critical role in embryonic development, tissue homeostasis, and cancer progression. This protein is applied in in vitro and in vivo assays to investigate pathway activation, cell differentiation, and tissue regeneration mechanisms. It is commonly used to stimulate canonical Wnt signaling in stem cell cultures, aiding in the maintenance of pluripotency or directing lineage-specific differentiation. Researchers also utilize it in developmental biology to explore kidney, limb, and reproductive system development, as WNT9A is known to regulate patterning and morphogenesis in these tissues. Its tag-free form ensures native conformation and optimal interaction with cell surface receptors, making it ideal for functional studies and receptor-binding assays.