Ergosterol peroxide is widely studied for its significant biological activities, making it valuable in pharmaceutical and nutraceutical applications. It exhibits strong anti-inflammatory effects by inhibiting pro-inflammatory mediators such as nitric oxide and cytokines, suggesting potential use in treating chronic inflammatory diseases. The compound also demonstrates notable anticancer properties, inducing apoptosis in various cancer cell lines including liver, lung, and breast cancer, which positions it as a candidate for anticancer drug development. Additionally, ergosterol peroxide has antimicrobial and antifungal activities, useful in combating pathogenic microorganisms. Its antioxidant capacity helps in reducing oxidative stress, supporting its role in protective therapies against degenerative diseases. Due to these diverse bioactivities, ergosterol peroxide is increasingly explored as a functional ingredient in health supplements and medicinal formulations, especially those derived from fungal sources.