Widely used in oligonucleotide synthesis, this compound enables the site-specific incorporation of 5-iodouridine into DNA strands. The iodine moiety acts as a handle for further functionalization, such as coupling via palladium-catalyzed cross-coupling reactions, allowing the attachment of labels, probes, or other functional groups. Its DMT and CE phosphoramidite protecting groups ensure compatibility with standard solid-phase synthesis protocols, making it ideal for automated DNA synthesizers. It is particularly valuable in the development of modified antisense oligonucleotides, diagnostic probes, and nucleic acid-based therapeutics where precise modification is required. The presence of the iodine atom also facilitates structural studies using techniques like X-ray crystallography or as a radiosensitizer in photodynamic therapy research.