Used in bioorthogonal chemistry for rapid and selective labeling of biomolecules due to its high reactivity in inverse electron-demand Diels-Alder reactions with strained alkynes. Commonly applied in live-cell imaging, where it enables real-time tracking of cellular components without interfering with biological processes. Its iodine substituent allows for further functionalization via radioiodination or cross-coupling reactions, making it valuable in radiopharmaceutical development, targeted molecular imaging, and targeted drug delivery systems, particularly in cancer research. Also employed in materials science for constructing functionalized surfaces and responsive nanomaterials through click chemistry approaches.