Dibenzoyl-L-tartaric acid is widely used in the pharmaceutical industry as a chiral resolving agent. It is particularly effective in separating enantiomers of racemic mixtures, which is crucial for producing optically pure compounds. This chemical is often employed in the synthesis of active pharmaceutical ingredients (APIs) where specific stereochemistry is required for efficacy and safety.

In addition to its pharmaceutical applications, it serves as a catalyst or additive in asymmetric synthesis, enhancing the selectivity and yield of chiral products. It is also utilized in research laboratories for studying stereochemistry and developing new chiral compounds.

Furthermore, Dibenzoyl-L-tartaric acid finds use in the food and beverage industry as a chiral selector for analyzing and separating enantiomers in complex mixtures. Its ability to interact selectively with specific enantiomers makes it valuable for quality control and ensuring the purity of chiral substances in various products.