N-Formylglycine plays a significant role in biochemical research, particularly in the study of enzyme mechanisms. It is a key intermediate in the catalytic cycle of sulfatases, enzymes that hydrolyze sulfate esters. Researchers utilize N-Formylglycine to understand and manipulate the activity of these enzymes, which are crucial in various biological processes, including the metabolism of hormones and the degradation of complex carbohydrates. Additionally, it is used in the development of enzyme inhibitors and in the design of therapeutic agents targeting sulfatase-related diseases. Its application extends to the field of synthetic biology, where it aids in the engineering of enzymes with enhanced or altered functions for industrial and medical purposes.