Used in biochemical research as an intermediate in carbohydrate metabolism, particularly in studies of glycolysis and phosphorylated sugar derivatives. It regulates key glycolytic enzymes such as phosphofructokinase and pyruvate kinase by acting as a phosphate donor. Serves as a substrate or analog in enzyme assays for kinases and phosphatases involved in phosphate group transfer, aiding in the understanding of cellular energy pathways. The hydrate form improves solubility and stability in aqueous environments, ideal for in vitro studies. Commonly employed as an analytical standard in techniques like HPLC and mass spectrometry for identifying metabolites in biological samples.