Used primarily as a coupling agent in bioconjugation, this compound enables the stable attachment of highly fluorinated tags or probes to biomolecules such as proteins, peptides, and antibodies. Its N-hydroxysuccinimide (NHS) ester group reacts efficiently with primary amines under mild conditions, forming stable amide bonds. The long perfluorinated chain provides exceptional hydrophobicity and chemical stability, making it ideal for applications in fluorine-19 MRI (19F MRI) imaging, where it acts as a sensitive tracer due to strong 19F NMR signals. It is also employed in the development of fluorinated surfaces for biosensors and diagnostic assays, where low background interference and high detection sensitivity are critical. Additionally, its unique lipophobic and hydrophobic characteristics are leveraged in creating non-fouling coatings and in studying molecular interactions in complex biological environments.