Used extensively in biomedical research to study cholesterol metabolism and its role in various diseases, particularly atherosclerosis. It serves as a biomarker for assessing cholesterol levels and understanding the mechanisms of lipid regulation in cells. Additionally, it is employed in investigating the effects of oxysterols on immune responses and inflammation. In drug development, it aids in evaluating potential therapeutic agents targeting cholesterol-related disorders. Its application extends to studying cellular signaling pathways and membrane dynamics influenced by cholesterol derivatives.