Allocholic acid is primarily used in biochemical research to study bile acid metabolism and its role in digestive processes. It serves as a reference compound in analytical studies to identify and quantify bile acids in biological samples. Additionally, it is utilized in the development of therapeutic agents targeting bile acid-related disorders, such as cholestasis and gallstones. Its applications also extend to investigating the interactions between bile acids and gut microbiota, which is crucial for understanding metabolic health and disease.