Benzamil hydrochloride is primarily used in scientific research to study ion channels, particularly sodium channels, in various cellular processes. It acts as a potent inhibitor of the epithelial sodium channel (ENaC), making it valuable in understanding fluid and electrolyte balance in tissues. This compound is often employed in experimental models to investigate conditions like hypertension, cystic fibrosis, and other disorders related to sodium transport. Additionally, it is utilized in pharmacological studies to explore its potential therapeutic effects in managing diseases associated with abnormal sodium channel activity. Its ability to block sodium uptake also makes it a useful tool in studying cellular mechanisms in renal and pulmonary systems.