Trypsin-chymotrypsin inhibitor from Glycine max (soybean) is primarily used in biomedical research and therapeutic applications. It is employed to study protease activity, as it effectively inhibits trypsin and chymotrypsin enzymes, which are involved in protein digestion and various cellular processes. This inhibitor is also utilized in developing anti-inflammatory and anticancer therapies, as protease inhibition can modulate pathways linked to disease progression. In the food industry, it is explored for its potential to improve nutritional value by reducing protease activity in soybean-based products. Additionally, it serves as a tool in biochemical assays to understand enzyme kinetics and protein interactions.