Benzamidine hydrochloride hydrate is widely used as a protease inhibitor in biochemical research. It effectively inhibits enzymes like trypsin, thrombin, and other serine proteases, making it valuable in studying enzyme mechanisms and protein interactions. In medical research, it aids in investigating blood coagulation pathways and developing therapeutic agents for clotting disorders. Additionally, it is utilized in cell culture studies to prevent protein degradation, ensuring the integrity of experimental results. Its role in stabilizing proteins and enzymes also makes it useful in diagnostic assays and pharmaceutical development.