Used primarily as a serine protease inhibitor in biochemical and molecular biology research. It effectively inactivates enzymes by covalently modifying active site serine residues. Commonly employed in cell lysis buffers and protein extraction protocols to prevent protein degradation during sample preparation. Also utilized in proteomics studies to stabilize protein samples and maintain integrity of labile post-translational modifications. Its selectivity and irreversible mode of action make it valuable in enzyme activity assays and inhibitor screening.