Used in microbiology as a selective and differential medium for the isolation and identification of Clostridium perfringens from clinical, food, and environmental samples. Supports the growth of anaerobic bacteria while inhibiting contaminants through the inclusion of specific antimicrobials. Colonies of C. perfringens typically appear as dark gray with a black halo due to sulfite reduction, allowing for easy visual identification. Commonly applied in food safety testing to detect spore-forming pathogens in meat, dairy, and processed foods. Also utilized in clinical labs for diagnosing clostridial infections, such as gas gangrene and food poisoning. The medium’s composition enhances spore germination and provides clear biochemical differentiation based on fermentation and reduction reactions.