Used in microbiology laboratories as a selective and differential medium for the cultivation and isolation of anaerobic bacteria, particularly in clinical specimens. It supports the growth of fastidious organisms and helps inhibit contaminants due to its alkaline pH. Commonly used in the diagnosis of infections involving anaerobes, such as those from the gastrointestinal tract, female genital tract, and deep wound infections. Also employed in research settings for studying bacterial metabolism under alkaline conditions.