Manganese chloride buffer is primarily used in molecular biology and biochemistry for various enzymatic reactions and protein studies. It is often employed as a stabilizing agent in PCR (Polymerase Chain Reaction) to enhance the activity of DNA polymerases, particularly in cases where the enzyme requires manganese ions for optimal function. Additionally, it is utilized in the study of metalloproteins and enzymes that rely on manganese as a cofactor, aiding in the investigation of their structure and function. The buffer is also applied in cell culture experiments to maintain ionic balance and support cellular processes that depend on manganese. Furthermore, it plays a role in certain diagnostic assays where manganese ions are necessary for accurate detection and measurement of specific biomarkers.