Used as a fluorescent dye in biological staining and cell imaging due to its strong lipophilic properties, allowing it to integrate into cell membranes. Commonly employed in mitochondria labeling because it accumulates in the mitochondrial matrix driven by the membrane potential. Also utilized in tracking membrane dynamics, such as fusion and fission events, in live cells. Its perchlorate salt form enhances solubility in organic solvents, making it suitable for preparing stock solutions in non-aqueous environments. Applied in neuroscience research for neuronal tracing and monitoring synaptic activity. Additionally, serves as a marker in liposome and vesicle studies to visualize membrane behavior under microscopy.