Endonuclease MnII is widely used in molecular biology for DNA manipulation and analysis. It specifically cleaves DNA at recognition sites, making it valuable for restriction mapping and genetic engineering. This enzyme is employed in cloning experiments to create precise DNA fragments for insertion into vectors. It also aids in studying DNA-protein interactions by generating defined DNA segments. Additionally, Endonuclease MnII is utilized in diagnostic applications, such as identifying genetic mutations or polymorphisms through restriction fragment length polymorphism (RFLP) analysis. Its specificity and efficiency make it a crucial tool in genetic research and biotechnology.