Used in molecular biology for DNA manipulation, particularly in restriction enzyme digestion. It recognizes specific DNA sequences and cuts them, enabling precise DNA fragment analysis. Commonly applied in cloning, genetic mapping, and recombinant DNA technology. It aids in studying gene structure, function, and regulation by facilitating DNA sequence modifications. Also utilized in diagnostic applications to identify genetic mutations or polymorphisms. Its specificity and efficiency make it a valuable tool in genetic engineering and biotechnology research.