Endonuclease HinfI is widely used in molecular biology for DNA analysis and manipulation. It is particularly valuable in restriction fragment length polymorphism (RFLP) studies, where it helps in identifying genetic variations by cleaving DNA at specific recognition sites. This enzyme is also employed in cloning experiments to create compatible DNA ends for ligation. Additionally, HinfI is utilized in genotyping and fingerprinting techniques to study genetic diversity and relationships among organisms. Its precision in cutting DNA makes it a crucial tool in genetic engineering and diagnostic applications.