Endonuclease CIaI is primarily used in molecular biology for its ability to cleave DNA at specific sites. It is particularly valuable in genetic engineering and cloning processes, where precise DNA manipulation is required. The enzyme is employed to create defined breaks in DNA strands, facilitating the insertion or removal of specific genetic sequences. This capability is crucial for constructing recombinant DNA molecules, which are essential for studying gene function, producing recombinant proteins, and developing genetically modified organisms. Additionally, Endonuclease CIaI is utilized in various diagnostic applications, including the detection of specific DNA sequences and the analysis of genetic mutations. Its specificity and efficiency make it a vital tool in advancing research and applications in biotechnology and medicine.