This compound is primarily used in biochemical research, particularly in the study of bacterial cell wall synthesis. It serves as a substrate analog for enzymes like D-alanyl-D-alanine carboxypeptidase and transpeptidase, which are involved in peptidoglycan formation. Researchers use it to investigate enzyme mechanisms and to screen for potential antibiotics that target bacterial cell wall biosynthesis. Additionally, it is employed in the development of antimicrobial agents, helping to understand resistance mechanisms and design inhibitors for drug-resistant bacteria. Its role in mimicking natural peptide substrates makes it valuable in enzymology and drug discovery studies.