DL-Xylose is commonly used in medical diagnostic tests, particularly in the diagnosis of malabsorption syndromes such as small intestinal bacterial overgrowth (SIBO) and celiac disease. When ingested, D-xylose is absorbed in the small intestine without being metabolized by bacteria under normal conditions. In a xylose absorption test, the patient consumes a xylose solution, and blood or urine levels are measured over time. Low levels indicate poor absorption, suggesting intestinal damage or dysfunction. It is also used in research settings to study carbohydrate metabolism and transport mechanisms in cells. Due to its structural similarity to glucose, DL-xylose can interfere with certain metabolic pathways, making it useful in investigating sugar transport proteins and enzyme specificity. In the food industry, DL-xylose serves as an intermediate in the production of xylitol, a sugar alcohol used as a low-calorie sweetener in sugar-free products like chewing gum, candies, and oral hygiene products. Its role in fermentation processes is explored for bio-based production of value-added chemicals such as xylonic acid and biofuels.