N-3-hydroxyoctanoyl-L-Homoserine lactone is an autoinducer involved in bacterial quorum sensing, a process of cell-to-cell communication that regulates gene expression, particularly in biofilm formation, virulence factor production, and environmental adaptation.

It is used in quorum sensing studies to understand bacterial communication and biofilm formation. Applied in research to develop anti-bacterial drugs targeting signaling pathways to control infections and inhibit pathogenesis. Utilized in synthetic biology to engineer microbial systems for industrial and environmental applications, such as preventing biofilm in water systems or medical devices.

Additionally, it is researched for agricultural applications to manage bacterial plant infections, potentially reducing pesticide use and supporting sustainable farming.