Methyl 2-Acetylpyridine-4-carboxylate is primarily used as a key intermediate in organic synthesis. It plays a significant role in the development of pharmaceuticals, particularly in the synthesis of compounds with potential therapeutic properties. Its structure is valuable for creating heterocyclic compounds, which are often explored for their biological activities, such as antimicrobial, anti-inflammatory, or anticancer effects.

Additionally, this chemical is utilized in the research and development of agrochemicals, where it contributes to the synthesis of novel pesticides or herbicides. Its reactivity and functional groups make it a versatile building block for designing complex molecules in both medicinal and agricultural chemistry. Researchers also employ it in the study of chemical reactions and mechanisms, aiding in the advancement of synthetic methodologies.