4-Ethynylpyridin-3-amine is primarily used in organic synthesis and pharmaceutical research. It serves as a key intermediate in the development of various bioactive compounds, particularly in the creation of molecules with potential therapeutic applications. Its ethynyl group allows for versatile chemical modifications, making it valuable in click chemistry reactions, which are widely employed in drug discovery and material science. Additionally, it is utilized in the synthesis of heterocyclic compounds, which are often explored for their antimicrobial, anticancer, and anti-inflammatory properties. Its pyridine moiety further enhances its utility in designing ligands for metal coordination and catalysis.