6-Aminopyrazolo[1,5-a]pyrimidine is primarily used in the field of medicinal chemistry as a key intermediate in the synthesis of various biologically active compounds. Its structure makes it a valuable building block for the development of drugs targeting specific enzymes and receptors.

One of its significant applications is in the design of kinase inhibitors, which are crucial in treating diseases like cancer, inflammation, and autoimmune disorders. The compound's pyrazolopyrimidine core is often incorporated into molecules that selectively inhibit kinases, helping to regulate cell signaling pathways.

Additionally, it is utilized in the development of antiviral and antimicrobial agents. Its structure allows for modifications that enhance binding affinity to viral or bacterial targets, making it a versatile scaffold for drug discovery.

In research, 6-Aminopyrazolo[1,5-a]pyrimidine is also employed in studying enzyme mechanisms and screening for potential therapeutic candidates due to its ability to interact with various biological targets. Its role in the synthesis of novel compounds continues to expand its applications in pharmaceutical development.