Z-Pyr-OtBu is widely used in peptide synthesis as a protecting group for carboxylic acids. It helps in preventing unwanted reactions during the formation of peptide bonds, ensuring the synthesis process is more controlled and efficient. This compound is particularly valuable in solid-phase peptide synthesis (SPPS), where it provides stability to the intermediate products. Additionally, it is employed in the preparation of complex organic molecules, where selective protection and deprotection of functional groups are crucial. Its application extends to medicinal chemistry, where it aids in the development of peptide-based drugs and bioactive compounds.