Benzyl 2-Acetamido-2-deoxy-β-D-glucopyranoside is primarily used in biochemical research and pharmaceutical development. It serves as a valuable intermediate in the synthesis of complex carbohydrates and glycoconjugates, which are essential for studying cell signaling, immune responses, and pathogen interactions. This compound is also utilized in the preparation of glycosidase inhibitors, which have potential therapeutic applications in treating diseases like diabetes and viral infections. Additionally, it plays a role in the development of glycoprotein-based drugs and vaccines, aiding in the enhancement of their stability and efficacy. Its application extends to the field of glycobiology, where it helps in understanding the structural and functional roles of carbohydrates in biological systems.