Z-Tyr-Tyr-OH is primarily utilized in biochemical research, particularly in the study of enzyme interactions and peptide synthesis. It serves as a substrate for investigating the activity of proteolytic enzymes, such as proteases, which cleave peptide bonds. Researchers use this dipeptide to understand enzyme specificity and kinetics, providing insights into metabolic pathways and protein function. Additionally, it is employed in the development of peptide-based drugs and as a building block in solid-phase peptide synthesis. Its structure makes it valuable for mimicking natural peptide sequences, aiding in the design of therapeutic agents targeting specific biological processes. In some cases, it is also used in the study of oxidative stress and cellular signaling pathways due to the presence of tyrosine residues, which are involved in phosphorylation and other post-translational modifications.