This compound is primarily utilized in peptide synthesis, particularly in solid-phase peptide synthesis (SPPS). It serves as a protected amino acid derivative, enabling the sequential addition of amino acids to build peptides with high precision. The Fmoc (fluorenylmethyloxycarbonyl) group acts as a temporary protecting group for the amine, which can be selectively removed under mild basic conditions, allowing for controlled elongation of the peptide chain. The tert-butyl (tBu) group protects the hydroxyl group of serine, while the pseudoproline moiety in the threonine residue enhances solubility and reduces aggregation during synthesis, improving the efficiency of peptide assembly. This compound is especially valuable in synthesizing complex peptides with specific secondary structures or for producing peptides used in research, drug development, and biochemical studies. Its application ensures high yield and purity in the final peptide product.