Boc-β-HoPro-OH is widely used in peptide synthesis as a protected form of hydroxyproline. It serves as a key building block in the creation of complex peptides, particularly those requiring the incorporation of hydroxyproline residues. This compound is essential in the development of collagen-like peptides, which are important in studies related to tissue engineering and biomaterials. Additionally, it is utilized in the synthesis of peptide-based drugs and bioactive molecules, where the protection of the hydroxyl group is necessary to prevent unwanted reactions during the synthesis process. Its role in ensuring the stability and specificity of peptide chains makes it a valuable tool in both academic research and pharmaceutical development.