N-(1-Naphthalenesulfonyl)-L-phenylalanine is primarily used in biochemical research as a substrate or inhibitor in enzymatic studies. It is particularly valuable in investigating the activity and specificity of proteases, as its structure allows it to interact with enzyme active sites. This compound is also utilized in the development of enzyme inhibitors for therapeutic purposes, aiding in the study of diseases where protease activity is dysregulated. Additionally, it serves as a building block in organic synthesis for creating more complex molecules with potential pharmacological applications. Its unique structure makes it a useful tool in understanding protein-ligand interactions and designing targeted drugs.