FMOC-NVA-OH is primarily used in peptide synthesis as a protecting group for amino acids. The FMOC group shields the amino group during the coupling process, ensuring selective reactions and preventing unwanted side products. It is particularly valuable in solid-phase peptide synthesis (SPPS), where it allows for the stepwise assembly of peptides with high efficiency and precision. After the desired peptide sequence is formed, the FMOC group can be easily removed under mild basic conditions, typically using piperidine, without affecting the peptide backbone. This makes FMOC-NVA-OH a critical reagent in the production of peptides for pharmaceutical research, drug development, and biochemical studies.