N-Tosylglycine is primarily used in organic synthesis as a versatile building block for the preparation of various biologically active compounds. It serves as a key intermediate in the synthesis of peptides and peptidomimetics, where the tosyl group acts as a protective group for the amino functionality. This compound is also employed in the development of pharmaceutical agents, particularly in the creation of inhibitors for enzymes and receptors. Additionally, N-Tosylglycine finds application in the study of biochemical pathways and as a reagent in the modification of proteins and peptides for research purposes. Its role in facilitating complex chemical reactions makes it valuable in medicinal chemistry and drug discovery.