L-Alanine benzyl ester hydrochloride is primarily used in the synthesis of peptides and other organic compounds. It serves as a protected form of alanine, where the benzyl ester group safeguards the carboxyl functionality during chemical reactions. This compound is particularly valuable in solid-phase peptide synthesis (SPPS), enabling the stepwise construction of peptides with high precision. Additionally, it is employed in the preparation of amino acid derivatives and as a building block in medicinal chemistry for developing pharmaceutical agents. Its stability and ease of deprotection make it a versatile reagent in biochemical and pharmaceutical research.