Used in peptide synthesis, particularly for the N-terminal protection of 5-chloro-DL-tryptophan during the formation of peptide bonds. It serves as a key intermediate in the production of complex peptides and proteins, enabling selective reactivity in multi-step chemical reactions. The 5-chloro modification on the indole ring of tryptophan provides a useful analog for studying structure-activity relationships in bioactive molecules. Additionally, it finds application in pharmaceutical research for developing bioactive peptides and drug candidates, where precise control over amino acid incorporation is crucial. Its protective Boc group can be selectively removed under mild acidic conditions, allowing for further modifications or coupling reactions.