Glycine tert-butyl ester hydrochloride is widely used in organic synthesis as a protecting group for carboxylic acids. It is particularly valuable in peptide synthesis, where it helps to prevent unwanted reactions at the carboxylic acid group during the formation of peptide bonds. This compound is also employed in the preparation of amino acid derivatives and intermediates for pharmaceutical applications. Its stability and ease of removal under mild conditions make it a preferred choice in complex synthetic pathways. Additionally, it finds use in the development of prodrugs, where it aids in enhancing the bioavailability and stability of active pharmaceutical ingredients.