This compound is primarily used in peptide synthesis, particularly in solid-phase peptide synthesis (SPPS). The Fmoc (fluorenylmethyloxycarbonyl) group serves as a protecting group for the amino group, ensuring selective reactions during the synthesis process. The N-methyl group on the serine residue introduces steric hindrance, which can influence the peptide's conformation and stability. This modification is often employed to enhance the peptide's resistance to enzymatic degradation, making it useful in the development of therapeutic peptides and peptidomimetics. Additionally, it is utilized in research to study the effects of N-methylation on peptide structure and function, aiding in the design of bioactive molecules with improved pharmacokinetic properties.