N-Formyl-L-valine is primarily used in the field of peptide synthesis. It serves as a protected form of valine, ensuring that the amino group remains intact during the synthesis process. This compound is particularly valuable in the production of complex peptides and proteins, where selective protection and deprotection of amino acids are crucial. Additionally, it finds applications in biochemical research, aiding in the study of peptide interactions and enzymatic processes. Its stability and compatibility with various synthetic methods make it a useful reagent in both academic and industrial settings.