H-Ala-oall Hydrochloride is primarily used in peptide synthesis as a building block for creating complex peptide structures. It serves as a protected amino acid derivative, ensuring specific reactions occur at desired sites without unwanted side reactions. This compound is particularly valuable in solid-phase peptide synthesis (SPPS), where it helps in the stepwise assembly of peptides. Its application extends to the development of pharmaceuticals, including peptide-based drugs, where precise control over amino acid incorporation is crucial. Additionally, it is utilized in research settings for studying peptide interactions, folding, and function, contributing to advancements in biochemistry and drug discovery.