O-Benzyl-L-tyrosine is widely used in peptide synthesis as a protected form of tyrosine. The benzyl group shields the hydroxyl group of tyrosine, preventing unwanted reactions during the peptide assembly process. This protection is crucial in solid-phase peptide synthesis, where selective deprotection is required to build complex peptide chains. Additionally, it serves as a key intermediate in the production of pharmaceuticals, particularly in the development of peptide-based drugs targeting various diseases. Its application extends to biochemical research, where it aids in studying enzyme mechanisms and protein interactions by providing a modified tyrosine residue for experimental purposes.