N-Boc-3-cyclohexyl-D-alanine is primarily used in the synthesis of peptides and peptidomimetics, where it serves as a protected amino acid derivative. The Boc (tert-butoxycarbonyl) group provides stability during peptide coupling reactions, ensuring the integrity of the amino acid structure. This compound is particularly valuable in medicinal chemistry for designing and developing peptide-based drugs, as the cyclohexyl group can enhance lipophilicity and influence the pharmacokinetic properties of the final molecule. It is also employed in research to study enzyme-substrate interactions and to create chiral building blocks for asymmetric synthesis. Its applications extend to the production of bioactive compounds targeting various diseases, including cancer and infectious diseases.