Used primarily in peptide synthesis as a protecting group for histidine residues. It helps prevent unwanted side reactions during the formation of peptide bonds. The Boc (tert-butyloxycarbonyl) group safeguards the amino group, while the Dnp (dinitrophenyl) group protects the imidazole side chain of histidine. This compound is particularly useful in solid-phase peptide synthesis (SPPS) to ensure precise and controlled assembly of peptides. It is also employed in the development of peptide-based drugs and biochemical research, where selective protection and deprotection of amino acids are crucial.